ASCP MB Practice Questions - Molecular Biology Technologist MB(ASCP) Exam

What does the Ct value (cycle threshold) represent in real-time PCR?

The total number of cycles in the PCR program The cycle number at which the fluorescence signal crosses a threshold above background, inversely proportional to the initial target quantity The temperature of the annealing step The concentration of primers used

Correct Answer:

The cycle number at which the fluorescence signal crosses a threshold above background, inversely proportional to the initial target quantity

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