Question 1
What is the principle of polymerase chain reaction (PCR)?
Correct Answer:
Enzymatic amplification of specific DNA sequences through repeated cycles of denaturation, annealing, and extension
Question 2
What is the function of Taq DNA polymerase in PCR?
Correct Answer:
It is a heat-stable DNA polymerase that synthesizes new DNA strands during the extension step of PCR
Question 3
What does the Ct value (cycle threshold) represent in real-time PCR?
Correct Answer:
The cycle number at which the fluorescence signal crosses a threshold above background, inversely proportional to the initial target quantity
Question 4
What is the purpose of reverse transcriptase in RT-PCR?
Correct Answer:
To convert RNA to complementary DNA (cDNA) before PCR amplification, enabling detection of RNA targets
Question 5
What is the difference between DNA and RNA in terms of structure?
Correct Answer:
DNA is double-stranded with deoxyribose sugar and thymine; RNA is single-stranded with ribose sugar and uracil
Question 1
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Prepare with the ASCP MB Practice Questions - Molecular Biology Technologist MB(ASCP) Exam practice quiz. This question bank includes 100 questions covering molecular, testing, significance, diagnostics, and principle. Use it to review important concepts, identify knowledge gaps, and build confidence for the related exam, course, or assessment.

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ASCP MB Practice Questions - Molecular Biology Technologist MB(ASCP) Exam

This practice set contains 100 questions from the matching question bank and focuses on molecular, testing, significance, diagnostics, and principle. Work through each question carefully, review the provided solutions, and revisit topics that need more study before your next attempt.

This is an independent study resource intended for practice and review; it is not an official examination or an endorsement by any organization named in the title.

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