Question 1
Which laboratory techniques are commonly used to genotype the Eda locus in sticklebacks?
Correct Answer:
PCR amplification of the Eda region followed by sequencing or SNP genotyping assays.
Explanation:
Genotyping a single gene locus like Eda is most efficiently done with targeted DNA analysis: amplify the Eda region by PCR and determine the genotype from the amplicon either by sequencing to read the exact variant(s) or by using a SNP-genotyping assay that detects known Eda variants. This direct approach zeroes in on the exact genetic differences that influence armor plating in sticklebacks, making it fast, accurate, and scalable to many individuals. Whole-genome sequencing of every sample, while informative, is much more resource-intensive and unnecessary when you only need the genotype at this one locus. Visual assessment of armor plates cannot reveal the underlying DNA sequence, and Southern blotting of random genomic regions isn’t a reliable or practical way to call genotypes at a specific locus.
Question 2
What is one key rationale for using sticklebacks as a model for studying rapid adaptation?
Correct Answer:
They show repeated, predictable evolutionary responses in multiple independent populations within short times, making them a powerful system for evolution in action.
Explanation:
The main idea here is that sticklebacks are a powerful model for rapid adaptation because they show repeated, predictable evolutionary changes in many independent populations within a short time frame. This parallel evolution occurs when freshwater sticklebacks repeatedly evolve similar traits—like reduced armor plates and pelvic girdles—despite starting from related marine populations. The consistency of these changes across different populations reveals natural selection acting under similar ecological pressures, making it possible to study both the phenotypic shifts and their genetic underpinnings, often in just a few generations. In this system, researchers can link quick changes in visible traits to specific genetic changes, explore how different populations converge on the same solutions, and compare how similar environments drive similar outcomes. The fact that these evolutions happen repeatedly and quickly, across many independent populations, makes sticklebacks an especially informative model for understanding evolution in action. The other statements don’t fit because the genome is not simply trivial to analyze, sticklebacks do respond to selection (they are classic examples of rapid adaptive change), and they are not restricted to laboratories—they are found in wild freshwater and marine environments worldwide.
Question 3
Why is the stickleback a model organism for studying evolution?
Correct Answer:
They show repeated recent evolution in postglacial lakes
Explanation:
This question is about showing evolution in action through repeated natural experiments. Sticklebacks are powerful model organisms because they undergo rapid, repeated evolutionary changes after shifting from marine to freshwater environments in postglacial lakes. In many independent lake populations, they converge on similar traits—like reduced armor plating and changes in pelvic structures—despite being geographically separate. This parallel evolution provides a clean, natural setup to compare how selection acts and how genetic variation is used to produce adaptive traits, all within short timescales that we can study directly. The repeated, predictable patterns across many populations make them ideal for uncovering how evolution proceeds in response to ecological change. Regarding the other statements: sticklebacks do have genetic variation and do not rely on ancient fixed traits; their ongoing adaptation is well documented. Fossil records exist for the lineage, but the strongest evidence for their use as a model comes from contemporary populations and genetic data showing repeated, parallel evolution.
Question 4
What would be a basic experimental approach to test if Eda variation causally affects plate number?
Correct Answer:
Create individuals with swapped Eda alleles using genome editing and compare their plate numbers.
Explanation:
This question is about proving causality by directly manipulating the genetic variant and watching the effect on the trait. The strongest way to test if a variation in Eda causes changes in plate number is to swap the Eda allele between individuals using genome editing and then compare plate numbers in a shared genetic background. By editing only the Eda allele and keeping all other genes and the environment the same, any difference in plate number can be attributed to the specific Eda variant. If the swapped allele leads to a different plate count, that provides direct evidence that Eda variation drives the trait. Observing natural populations or comparing knockouts alone introduces confounds. A cross between species or transferring Eda across backgrounds brings many uncontrolled genetic differences and environmental factors, muddying the link between Eda variant and plate number. A knockout shows whether Eda is necessary for the trait, but it doesn’t test how natural allelic differences influence plate number in a controlled background.
Question 5
What is a 'ghost of selection' concept in evolutionary genetics?
Correct Answer:
Past selection leaves detectable signatures in the genome, such as elevated allele frequencies or reduced diversity, even after selection ends. D. It explains why selection cannot leave any genomic signature.
Explanation:
The main idea is that historical selection can leave lasting traces in the genome that stick around even after the selective pressure has ended. When a beneficial allele sweeps through a population, nearby variants can hitchhike with it, creating a sweep that reduces genetic diversity and creates extended regions of linkage. After selection ends, recombination gradually erodes these patterns, but remnants—like unusually high frequencies of certain alleles and lower diversity in the surrounding region—can persist as a “ghost” of the past selection. Those genomic signatures let us infer that adaptation occurred in the past, even though the current conditions no longer favor that allele. So the best description is that past selection leaves detectable signatures in the genome, such as elevated allele frequencies or reduced diversity, even after selection ends. The other options don’t capture this idea: phenotypic changes disappearing isn’t about lingering genomic traces, the notion that ghosts cause spurious signals misstates the concept, and it’s false to say selection leaves no genomic signature at all.
Question 1
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Prepare with the Stickleback Practice Test practice quiz. This question bank includes 10 questions covering sticklebacks, stickleback, commonly, model, and studying. Use it to review important concepts, identify knowledge gaps, and build confidence for the related exam, course, or assessment.

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Stickleback Practice Test

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