Question 1
Thayer-Martin medium is used to isolate which organism?
Correct Answer:
Neisseria gonorrhoeae
Explanation:
Thayer-Martin medium is a selective culture medium designed to isolate Neisseria species, especially Neisseria gonorrhoeae. It uses a chocolate agar base enriched for Neisseria growth and includes antibiotics such as vancomycin to suppress Gram-positive bacteria, colistin to inhibits many Gram-negative rods, and nystatin to prevent fungal growth. This combination reduces competing flora found in clinical specimens, allowing Neisseria gonorrhoeae to grow when present. Neisseria gonorrhoeae is a fastidious Gram-negative diplococcus that causes gonorrhea, so this selective medium is particularly useful for recovering it from samples like urethral or cervical swabs. The other organisms listed are typically inhibited by these antibiotics, so they would not be favored growth on this medium.
Question 2
The greatest antigen-antibody reaction occurs under which transfusion circumstance?
Correct Answer:
A person with type O blood is given type A blood.
Explanation:
The main idea is that transfusion reactions happen when antibodies in the recipient attack antigens on the donor’s red blood cells. In the ABO system, type O individuals have antibodies against both A and B antigens in their plasma. If a type O recipient receives type A blood, the anti-A antibodies in the recipient will bind to the A antigens on the donor red cells. This triggers strong agglutination and hemolysis, producing the largest immune response. In the other scenarios, the donor cells either lack the target antigens or the recipient lacks antibodies against those antigens, so the reaction is much less pronounced.
Question 3
Which test is typically performed in the Microbiology section?
Correct Answer:
Gram stain
Explanation:
In Microbiology, a Gram stain is a foundational, rapid test used to categorize bacteria by their cell wall properties. It distinguishes Gram-positive organisms, which retain the crystal violet-iodine stain and appear purple, from Gram-negative organisms, which do not retain it and appear pink or red after a counterstain. This quick result guides early identification and helps inform initial treatment decisions while more specific tests and cultures are being processed. The other options—CBC, lipid panel, and electrolytes—belong to hematology and chemistry sections and measure blood cells or metabolic parameters, not microbial characteristics.
Question 4
Which structure of the heart delivers oxygen-rich blood to the ascending aorta?
Correct Answer:
Left Ventricle
Explanation:
The left ventricle is the chamber that delivers oxygen-rich blood into the ascending aorta. After blood is oxygenated in the lungs, it returns to the heart and enters the left atrium, moves through the mitral valve into the left ventricle, and then the ventricle contracts to push blood through the aortic valve into the ascending aorta. The left ventricle has a thick muscular wall to generate the high pressure needed to supply the entire systemic circulation. The other chambers handle blood in the right side of the heart or receive blood from the body and lungs, but they do not eject oxygen-rich blood into the ascending aorta.
Question 5
All the following tubes may contain EDTA as an anticoagulant, EXCEPT:
Correct Answer:
Gray-Orange Speckled Tube
Explanation:
EDTA prevents coagulation by binding calcium, which is why it's used in tubes for hematology tests like the CBC. Pink-top tubes are specifically EDTA-containing for hematology/immunohematology. Royal blue tops are often used with EDTA for trace-element and molecular tests, and white-top tubes are commonly EDTA-containing for DNA/RNA work. The gray-orange speckled tube, however, is designed for chemistry testing and uses a different additive combination (typically fluoride/oxalate) rather than EDTA to preserve glucose and prevent clotting. Because it does not rely on EDTA, it’s the exception among tubes that may contain EDTA.
Question 1
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Prepare with the PMLS 2 Comprehensive Practice Test practice quiz. This question bank includes 10 questions covering blood, capillary, specimens, left, and pmls. Use it to review important concepts, identify knowledge gaps, and build confidence for the related exam, course, or assessment.

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PMLS 2 Comprehensive Practice Test

This practice set contains 10 questions from the matching question bank and focuses on blood, capillary, specimens, left, and pmls. Work through each question carefully, review the provided solutions, and revisit topics that need more study before your next attempt.

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