PMLS 2 Comprehensive Practice Test

All the following tubes may contain EDTA as an anticoagulant, EXCEPT:

Pink-top tube Royal blue-top tube Gray-Orange Speckled Tube White-top tube

Correct Answer:

Gray-Orange Speckled Tube

Explanation:

EDTA prevents coagulation by binding calcium, which is why it's used in tubes for hematology tests like the CBC. Pink-top tubes are specifically EDTA-containing for hematology/immunohematology. Royal blue tops are often used with EDTA for trace-element and molecular tests, and white-top tubes are commonly EDTA-containing for DNA/RNA work. The gray-orange speckled tube, however, is designed for chemistry testing and uses a different additive combination (typically fluoride/oxalate) rather than EDTA to preserve glucose and prevent clotting. Because it does not rely on EDTA, it’s the exception among tubes that may contain EDTA.

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