DAT Bootcamp Molecules And Fundamentals Of Biology Practice Test

What are the two ends of DNA?

5' end and 3' end 3' end only 5' end only None of the above

Correct Answer:

5' end and 3' end

Explanation:

DNA has directionality: its sugar-phosphate backbone is built from 5' to 3' as nucleotides connect from the 5' phosphate of one nucleotide to the 3' hydroxyl of the next. This creates a free 5' phosphate at one end and a free 3' hydroxyl at the other end. In a double-stranded molecule, each strand carries these ends, so the molecule has both a 5' end and a 3' end. Therefore, the two ends are the 5' end and the 3' end. The other options imply only one end or none, which isn’t accurate.

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