Praxis Biology 5236 Practice Questions - Praxis Biology Content Knowledge (5236) Exam

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A student measures enzyme activity (in units per minute) at five temperatures and creates a graph. The curve rises steeply, peaks at 37°C, then drops sharply at higher temperatures. The drop above 37°C is most likely due to:

Substrate depletion as the reaction proceeds too quickly Enzyme denaturation causing the active site shape to change permanently The reaction reaching chemical equilibrium at high temperatures CO₂ production inhibiting the enzyme's active site

Correct Answer:

Enzyme denaturation causing the active site shape to change permanently

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