DABCC Practice Questions - Diplomate Of The American Board Of Clinical Chemistry Exam

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In high-performance liquid chromatography (HPLC) using a reversed-phase C18 column, which analyte characteristic causes the longest retention time?

High polarity and water solubility High hydrophobicity (nonpolar character) High ionic charge at the mobile-phase pH Low molecular weight only

Correct Answer:

High hydrophobicity (nonpolar character)

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