ABC Forensic DNA Practice Questions - ABC Forensic Biology Molecular Biology Certification (Specialty Exam) Exam

An analyst uses Chelex 100 for DNA extraction. Which statement best describes the mechanism and the resulting DNA?

Chelex chelates magnesium ions and denatures cellular proteins, yielding single-stranded DNA suitable for PCR Chelex precipitates DNA in ethanol, yielding purified double-stranded DNA Chelex binds DNA to magnetic beads, yielding column-purified DNA Chelex digests proteins via proteinase K only, yielding RNA-rich extract

Correct Answer:

Chelex chelates magnesium ions and denatures cellular proteins, yielding single-stranded DNA suitable for PCR

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