Higher Human Biology Practice Questions - Higher Human Biology (Qualifications Scotland SCQF Level 6) Exam

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During DNA replication in human cells, why is a short RNA or DNA primer required before DNA polymerase can begin synthesis?

DNA polymerase requires an existing 3' hydroxyl group to attach the first incoming deoxyribonucleotide The primer unwinds the hydrogen bonds holding the parental template strands together DNA polymerase cannot recognize purine bases without a primer bound to the 5' end The primer acts as an enzyme that ligates Okazaki fragments on the leading strand

Correct Answer:

DNA polymerase requires an existing 3' hydroxyl group to attach the first incoming deoxyribonucleotide

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