SACE Stage 2 Biology Practice Questions - SACE Stage 2 Biology External Assessment Exam

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In Meselson and Stahl's classic experiment, E. coli cultured in 15N (heavy nitrogen) were transferred to 14N (light nitrogen) medium for two generations of cell division. What density gradient band pattern confirms semi-conservative replication after generation 2?

100% heavy (15N/15N) band 50% intermediate hybrid (15N/14N) band and 50% light (14N/14N) band 100% intermediate hybrid (15N/14N) band 75% heavy (15N/15N) band and 25% light (14N/14N) band

Correct Answer:

50% intermediate hybrid (15N/14N) band and 50% light (14N/14N) band

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