Chromatography Practice Test

If two adjacent peaks exhibit Rs of 2.0, what does this indicate?

They are baseline separated. They are partially overlapping. They have the same retention time. They are not detected.

Correct Answer:

They are baseline separated.

Explanation:

Resolution between two adjacent peaks is a measure of how well they are separated in chromatography. It combines how far apart their retention times are and how wide the peaks are. An Rs value of 2.0 indicates good, baseline separation: there is a clear baseline between the two peaks, so they do not overlap and you can quantify each peak independently. If peaks were partially overlapping, the Rs would be lower, not 2.0. If they had the same retention time, they would co-elute and the separation would be effectively nonexistent. If they were not detected, there would be no peaks to measure at all. So, a value of 2.0 means the peaks are baseline separated.

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