SMB Practice Questions - ASCP Specialist In Molecular Biology Exam

During PCR, what occurs at the denaturation step (typically 94-98 degrees C)?

Double-stranded DNA separates into single strands by breaking hydrogen bonds Primers anneal to complementary template sequences Taq polymerase extends primers using dNTPs Final products are stabilized by covalent bond formation

Correct Answer:

Double-stranded DNA separates into single strands by breaking hydrogen bonds

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