Toelatingsexamen Dierenarts Practice Questions - Toelatingsexamen Dierenarts - Flanders Veterinary Medicine (diergeneeskunde) Bachelor Admission Exam

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A clinic enzyme assay on horse plasma is run at increasing temperature while substrate is in excess. What happens to the initial reaction rate as temperature rises from well below the enzyme’s optimum to well above it?

The rate falls continuously because enzymes are inactivated by any temperature increase The rate is independent of temperature and is set only by the amount of cofactor The rate doubles for every 1 °C rise at all temperatures, including above the optimum The rate increases up to an optimum and then falls as the protein denatures

Correct Answer:

The rate increases up to an optimum and then falls as the protein denatures

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