ABMGG Molecular Genetic Pathology Practice Questions - Molecular Genetic Pathology (ABMGG) Exam

In quantitative real-time PCR, the cycle threshold (Ct) value is BEST defined as:

The cycle at which fluorescence crosses a defined threshold above background The maximum number of cycles allowed before plateau The cycle at which primer-dimer signal equals target signal The cycle at which DNA polymerase becomes saturated

Correct Answer:

The cycle at which fluorescence crosses a defined threshold above background

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