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National Center For Competency Testing (NCCT) Practice Exam

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What is the CLSI recommended micro-collection order of draw for a BMP, CBC and bilirubin?
Correct Answer:
Lavender, red, green
Explanation:
The idea being tested is how to order draws so additives in one tube don’t contaminate the others. Lavender tubes contain EDTA for the CBC and protect cell integrity, red tubes are serum tubes with no anticoagulant, and green tubes contain heparin for plasma chemistry like a BMP. Following the CLSI micro-collection guideline, drawing CBC first in the EDTA tube, then bilirubin in a red serum tube, and finally BMP in a green heparin tube minimizes the chance that EDTA or other additives carry over into the chemistry samples. If the chemistry tubes were drawn sooner, EDTA or heparin could contaminate the serum or plasma samples and distort results, especially in chemistry measurements and calcium-related assays.

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