MLT Registration Practice Questions - Medical Laboratory Technologists Board Of Hong Kong Registration Assessment Exam

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Which of the following describes the chemical basis of the classic Jaffe reaction for measuring creatinine, and what is a common positive interferent?

Reaction with copper sulfate in alkaline solution; interfered with by lipids Reaction with picric acid in alkaline solution; interfered with by acetoacetate Enzymatic oxidation by creatinine amidohydrolase; interfered with by uric acid Reaction with sodium nitroprusside in acid solution; interfered with by glucose

Correct Answer:

Reaction with picric acid in alkaline solution; interfered with by acetoacetate

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