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MCAT Bio Biochem Practice Questions - MCAT Biological And Biochemical Foundations Of Living Systems Exam

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Passage: A researcher studies a mutant enzyme that converts substrate S to product P. Kinetic data collected at varying [S] with fixed enzyme concentration are shown below: [S] (mM) | Initial velocity (µM/min) 0.5 | 15 1.0 | 25 2.0 | 37 5.0 | 48 10.0 | 53 The researcher estimates Vmax ≈ 60 µM/min from a Lineweaver-Burk plot. What is the approximate Km of this enzyme?
Correct Answer:
2.0 mM

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