Life Science MISA Practice Questions - Maryland Integrated Science Assessment - Life Science Exam

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An enzyme-catalyzed reaction rate was measured across temperatures from 10°C to 70°C. The reaction rate increased up to 37°C, peaked, and sharply dropped to zero above 55°C. What explains the drop in activity above 55°C?

Substrate molecules were destroyed by thermal energy. The enzyme's tertiary active site denatured due to broken hydrogen bonds. The activation energy of the reaction decreased to zero. Competitive inhibitors formed spontaneously at elevated temperatures.

Correct Answer:

The enzyme's tertiary active site denatured due to broken hydrogen bonds.

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