JLP Phlebotomy Practice Exam

For blood spot collection in newborns, what must be done before testing?

The specimen must be completely dried before processing Refrigerate immediately Freeze before processing Use a wet sample

Correct Answer:

The specimen must be completely dried before processing

Explanation:

Drying the blood spot completely before testing is essential. Dried spots on filter paper are stable and ready for reliable extraction of analytes; moisture left in the sample can cause diffusion, dilute or degrade components, promote contamination, and lead to inaccurate test results. After collection, allow the spot to air-dry thoroughly at room temperature on a clean surface away from heat and humidity, following the program’s drying time, before proceeding with testing. Refrigerating or freezing before drying isn’t part of normal pre-test handling, and a wet sample isn’t acceptable.

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