CalRegional Phlebotomy Practice Test

Which antiseptic is most commonly used to cleanse the venipuncture site?

Hydrogen peroxide. Povidone-iodine. 70% isopropyl alcohol. Soap and water.

Correct Answer:

70% isopropyl alcohol.

Explanation:

Using a fast-acting, quickly drying antiseptic is essential for venipuncture. 70% isopropyl alcohol is preferred because it rapidly reduces skin microbes and dries quickly, leaving little residue that could interfere with the blood sample or tests. Hydrogen peroxide isn’t ideal for skin prep of puncture sites due to potential tissue irritation and because it’s not as effective for antisepsis on intact skin. Povidone-iodine takes longer to dry and can leave residues that affect certain tests or stain the skin. Soap and water cleans but does not provide ongoing antiseptic action at the puncture site. For these reasons, the most common choice is 70% isopropyl alcohol.

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