SMB Practice Questions - ASCP Specialist In Molecular Biology Exam

In ddCt (delta delta Ct) relative quantification, what does the second delta represent?

Difference between target and reference gene Ct in each sample Difference between sample dCt and calibrator (control) dCt Difference between technical replicates Difference between SYBR and TaqMan readings

Correct Answer:

Difference between sample dCt and calibrator (control) dCt

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